Webb2 mars 2013 · Expert opinion: When human (cryopreserved) hepatocytes are used to establish sandwich cultures, the model appears particularly valuable to quantitatively … WebbWe have developed and validated a biorelevant sandwich-cultured hepatocytes- (SCH) based model that can identify compounds causing cholestasis by altering bile acid disposition. Human and rat SCH were exposed (24-48 h) to known cholestatic and/or hepatotoxic compounds, in presence or in absence of a concentrated mixture of bile …
Comparison of Hepatic 2D Sandwich Cultures and 3D Spheroids
Webb30 okt. 2013 · The objective of this study was to compare the metabolism of T4 in untreated and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153)-treated primary sandwich-cultured hepatocytes from rat (SCRH) and human ... Webb14 okt. 2024 · In this study, hepatocytes were co-cultured with stellate cells in a microfluidic device that also contained miniature electrodes functionalized with TGF-β1-sepcific aptamer molecules. These co-cultures were injured by exposure to alcohol, and the production of TGF-β1 was monitored electrochemically. safe-o-tronic access ds
Brian Ferslew - Director - Clinical Pharmacology - LinkedIn
WebbSandwich-cultured hepatocytes maintain expression of liver-specific proteins and gradually form intact bile canaliculi with functional biliary excretion of endogenous … Webbcells cultured on amorphous collagen I between days 2 and 13, and the gradual loss in the number of cells cultured in collagen I gels. In contrast, hepatocytes cultured on Corning Matrigel Matrix maintain a constant cell number through day 22. 0.0 0.2 0.4 0.6 0.8 1.0 12 58 13 16 17 19 22 Days in Culture WebbHepatocytes cultured at a higher seeding density in a sandwich configuration exhibit an increased level of liver specific functions when compared to low density cultures due to the better cell to cell contacts that promote long term maintenance of … safe-o-tronic ls100