Psmart bac vestor
WebThe pSMART® BAC vectors incorporate transcription-free cloning to provide maximum stability. These vectors are supplied pre-cut at BamHI, EcoRI, or HindIII sites, with … WebApr 29, 2015 · The Lucigen pSMART BAC vector was modified to operate as a shuttle vector between E. coli and A. nidulans. The AMA1 DNA fragment, previously identified as an autonomously replicating DNA fragment from A. nidulans [ 22 ], was incorporated into pSMART BAC to create pSMART-BAC pyrGAMA1-4, or shuttle BAC vectors, and tested for …
Psmart bac vestor
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WebA similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. BACs are often used to sequence the genome of organisms in genome projects, for example the Human Genome Project. A short piece of the organism's DNA is amplified as an insert in BACs, and then sequenced. WebThese vectors incorporate the CloneSmart® transcription-free technology, which minimizes cloning bias and enables cloning of otherwise toxic genes, along with inducible copy number amplification (20-50X) for high DNA yields and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants, resulting in zero background.
WebCopyRight®v2.0 BAC Cloning Kit, pSMART® BAC HindIII with BAC-Optimized Replicator™v2.0 Electrocompetent Cells sufficient for 20 reactions; find Sigma-Aldrich-LUC420322 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Webrestriction mapping. Lucigen’s pSMART® or pEZ™ BAC vector is pre-cut and dephosphorylated, eliminating the need for vector preparation. CopyRight kits contain the …
WebThe pSMART-BAC vector was modified as described in Materials and Methods. The full-length genomic cDNA of PEDV was amplified by RT-PCR using seven overlapping … Web1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. …
WebJul 31, 2024 · The CopyRight pSMART BAC cloning vector harboring the chloramphenicol resistance gene was introduced into the AnpeNPV genome to produce the AnpeNPV bacmid that could be propagated in...
WebDec 9, 2024 · The pSMART BAC HindIII vector (7.6 kb) was fully digested and dephosphorylated by using HindIII restriction enzyme and calf intestinal alkaline phosphatase (CIAP) enzyme (NEB, UK) at 37 °C for 60 min. Afterwards, the partially digested oral metagenome was ligated into a pre-digested pSMART BAC vector by using Anza T4 … pearl chemicalsWebAug 1, 2024 · The CopyRight pSMART BAC cloning vector harboring the chloramphenicol resistance gene was introduced into the AnpeNPV genome to produce the AnpeNPV bacmid that could be propagated in Escherichia coli with stable replication. lighttech incWebJan 2, 2015 · The full-length RespPRRSV genome was assembled in vitro as illustrated in Fig. 1.Briefly, after gel clean-up, PCR products of both the modified pSMART BAC vector and the four viral cDNA fragments were assembled together in an isothermal GA reaction (Gibson et al., 2009).Fragments were combined with a home-made 1.3× GA master mix, … pearl chemicals ltd