Fosmid library production

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August undefined, 2024

WebAug 13, 2015 · Genomic library construction and screening. A genomic library was constructed with the CopyControl™ Fosmid Library Production Kit using the pCC2FOS vector and the phage T1-resistant E. coli EPI300 plating strain. Titering the packaged CopyControl fosmid clones indicated that the library titer was 1 × 10 4 CFU/ml. http://www.nwbiotec.com/index.php?g=asset&m=download&a=dw&smurl=5044

Preparation of fosmid libraries and functional …

WebCopyControl Fosmid Library Production Kits WebOct 8, 2013 · A fosmid library (average insert size, approximately 40 kb) was constructed via pCC 1FOS™ vector using the CopyControl™ fosmid library production kit then plating on plating Escherichia coli EPI300-T1 R strain, which represented 4×10 8 bp of metagenomic DNA. capture is disallowed since high-density

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WebJan 1, 2010 · Here, we describe one protocol for the construction of small-insert libraries and one for large-insert fosmid libraries. Both methods have been proven to be suitable for cloning of DNA purified from various environmental samples, including soil, ice, and compost ( 6 – 8 ). 2 Materials 2.1 Metagenomic DNA WebNov 26, 2024 · Fosmid library construction. Fosmid libraries were constructed using the method modified from the protocol of Copy Control™ HTP Fosmid Library Production … WebSep 3, 2014 · Product Description. The EpiFOS Fosmid Library Production Kit contains all reagents needed to construct a complete and unbiased fosmid library in about two days … britwind ltd

Copycontrol™ Fosmid Library Production Kit With Pcc1Fos™ Vector

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WebMay 11, 2012 · High molecular weight genomic DNA was used to clone the P-SSP7 genome into the pCC1FOS fosmid using the CopyControl Fosmid Library Production kit (Epicentre Biotechnologies) following the manufacturer’s instructions. Briefly, the phage DNA was treated with T4 DNA polymerase and T4 polynucleotide kinase to generate … Webhigh-quality fosmid DNA. The scalable protocol is based on a modified alkaline-lysis procedure that can be used with 1.5–100 mlof culture. Consistent yields of up to 0.6, 15 or 25 μg of fosmid DNA are obtained from 1.5, 40 or 100 ml cultures of a single-copy fosmid, respectively. Up to 4 µg of fosmid DNA can be isolated from 1.5 ml of capture images on screenWebSep 4, 2015 · Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteriaof,a,to,and ... CopyControlFosmid Library Production Kit pCC1FOSfosmid vector (Epicentre Biotechnologies, Madison, WI, USA) used.Because more than 10% eDNAhad averagesize 30–40kb, furtherpurification … britwres

"WebHere, we describe methods for isolating environmental DNA and constructing metagenomic fosmid libraries, as well as methods for designing and implementing successful … " - Fosmid library production

Preparation of fosmid libraries and functional …

Recovery and functional validation of hidden ... - Wiley Online Library

Fosmid library production

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