WebOct 18, 2024 · The ctEF-1α element of vector enhanced transcription and translation. Figure 2a shows the effect of the presence of the wild-type (wt) EF-1α first intron in the promoter, which results in a ... WebAccording to these findings, we concluded that the EF-1α promoter is a potent regulatory sequence for episomal vectors because it maintains high transgene expression, …

Why is there a difference between the EF1a promoter on …

WebIn this study, we investigated the effect of five different introns on transgene expression in CHO cells. hCMV intron A, adenovirus tripartite leader sequence intron, SV40 intron, Chinese hamster EF-1alpha gene intron 1 … WebIn order to isolate an elongation factor-1α (EF-1α)gene from cassava, a lambda EMBL3 genomic library, made from a single cassava genotype (MBRA 534, from the CIAT cassava germplasm collection), was screened with a full length EF-1α cDNA clone(blt63) from barley. Six positive clones were isolated from an amplified library and 4866 bp from one clone … grey and brown jansport backpack

Translation elongation factor 1-α gene as a ... - Oxford Academic

WebFeb 14, 2002 · The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. In order to develop a highly efficient mammalian expression vector, we constructed a vector … WebEF-1α they only noted the total number of sequences and the orders for which it was sequenced. This paper reviews the extent of sequenced regions in EF-1α, their exon-intron structures, and the presence of paralogous copies. Our purpose is to evaluate the phylogenetic utility of EF-1α and recommend a focal http://www.inovogen.com/lentivirus/lentivirusvector/pLV-EF1a-EGFP2APuro/ grey and brown pillows